Since October last year, the United States has banned non-vaccinated visitors.
The 21-time Grand Slam winner confirmed his absence via his official Twitter account.
He wrote: “Sadly, I will not be able to travel to NY this time for US Open. Thank you [fans] for your messages of love and support.
“Good luck to my fellow players! I’ll keep in good shape and positive spirit and wait for an opportunity to compete again.
“See you soon tennis world!”
The organizers of the US Open at Flushing Meadows have also released an official statement confirmed on Thursday, confirming the ban.
A statement via their official Twitter page read: “Novak Djokovic withdrew from the 2022 US Open prior to the draw taking place this morning.
“The official seeding list is not determined until the draw is made.
“As the Qualifying Tournament has commence , his withdrawal resulted in a Lucky Loser being included in the draw.”
However, the Serbian international will miss out on another chance at Flushing Meadows.